| 質粒名稱: | pBabe neo IRES-EGFP |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 14431 |
| 存儲實驗室: | L. Miguel Martins |
|
載體骨架基本信息 |
|
| 載體名稱: | Babe neo |
| 載體出品公司: | Clontech |
| 空載體大小: | 5200bp |
| 完整質粒大小: | 6644 bp |
| 載體類型: | 哺乳細胞表達, Retroviral |
| 篩選標記: | 新霉素（G418） |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 未知 |
|
插入基因信息 |
|
| 插入基因名稱: | IRES-EGFP |
| 物種來源: | Aequorea victoria |
| 克隆方法: | 限制性內切酶 |
| 5’克隆位點: | SnaB1, EcoRI (not destroyed) |
| 5’測序引物 | n/a |
|
文獻引用方法 |
|
| 材料和方法部分: | pBabe neo IRES-EGFP was a gift from L. Miguel Martins (Addgene plasmid # 14431 ; //n2t.net/addgene:14431 ; RRID:Addgene_14431) |
買家導航
pBabe neo IRES-EGFP載體質粒基本信息
pBabe neo IRES-EGFP質粒圖譜載體圖譜和pBabe neo IRES-EGFP載體序列質粒序列多克隆位點信息
pBabe neo IRES-EGFP質粒載體簡介
pBabe neo IRES-EGFP質粒序列載體序列
LOCUS pBabe neo IRES-EGFP 6644 bp DNA circular SYN 14-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6644)
TITLE pBabe-IRES-EGFP plasmids
REFERENCE 2 (bases 1 to 6644)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT Sequence Label: pBabe neo IRES-EGFP
FEATURES Location/Qualifiers
source 1..6644
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 8..477
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 542..741
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 934..1350
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1318..1334
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
misc_feature join(1425..1999,2000..2002,2003..2011)
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(1592..1609)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 1819..1838
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS join(2012..2014,2015..2017,2018..2731)
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS join(2012..2014,2015..2017,2018..2728)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2057..2078)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2318..2337)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2665..2686
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(2742..2762)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2747..3076
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2927..3062
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3433..4227
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3487..3506)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4097..4116
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 4718..4737
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
LTR 4876..5469
/note="long terminal repeat from Moloney murine leukemia
virus"
CDS complement(join(5656..6447,6448..6516))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6279..6298
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6517..6621)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga
1381 attcgccagc acagtggtcg acggtaccgc gggcccggga tccgcccctc tccctccccc
1441 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
1501 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
1561 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
1621 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
1681 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
1741 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
1801 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
1861 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
1921 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
1981 tgaaaaacac gatgataata tggccacaac catggtgagc aagggcgagg agctgttcac
2041 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
2101 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
2161 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
2221 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
2281 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
2341 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
2401 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
2461 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
2521 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
2581 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
2641 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
2701 cactctcggc atggacgagc tgtacaagta agtcgacctg tggaatgtgt gtcagttagg
2761 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
2821 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
2881 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
2941 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
3001 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
3061 cctaggcttt tgcaaaaagc ttcacgctgc cgcaagcact cagggcgcaa gggctgctaa
3121 aggaagcgga acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc
3181 agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc
3241 agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa
3301 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct
3361 ttcttgccgc caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatga
3421 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
3481 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
3541 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
3601 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
3661 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
3721 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
3781 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
3841 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
3901 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
3961 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
4021 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
4081 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
4141 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
4201 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttatcgatc gtaatctgct
4261 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
4321 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
4381 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
4441 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
4501 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
4561 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
4621 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
4681 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
4741 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
4801 ggcggagcct atggaaaaac gccagcaacg atcgataaaa taaaagattt tatttagtct
4861 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
4921 gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc
4981 aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
5041 ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt
5101 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag
5161 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
5221 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
5281 tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat
5341 tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
5401 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
5461 tctttcattt ccgacttgtg gtctcgctgc cttgggaggg tctcctctga gtgattgact
5521 acccgtcagc gggggtcttc acatgcagca tgtatcaaaa ttaatttggt tttttttctt
5581 aagtatttac attaaatggc catagtgcgg ccgcaaatca atctaaagta tatatgagta
5641 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
5701 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
5761 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
5821 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
5881 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
5941 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
6001 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
6061 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
6121 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
6181 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
6241 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
6301 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
6361 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
6421 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
6481 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
6541 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
6601 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//